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Efficient RNA 5′-adenylation by T4 DNA ligase to facilitate practical applications

We describe a simple procedure for RNA 5′-adenylation using T4 DNA ligase. The 5′-monophosphorylated terminus of an RNA substrate is annealed to a complementary DNA strand that has a 3′-overhang of 10 nucleotides. Then, T4 DNA ligase and ATP are used to synthesize 5′-adenylated RNA (5′-AppRNA), whic...

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Bibliografiske detaljer
Main Authors: Wang, Yangming, Silverman, Scott K.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1464850/
https://ncbi.nlm.nih.gov/pubmed/16618967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.33106
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