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Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase
We report a simple method of enzymatic synthesis of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl). Using RNA ligase for the reaction instead of the existing chemical or T4 DNA ligase-based methods a...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3177227/ https://ncbi.nlm.nih.gov/pubmed/21724605 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr544 |
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