Loading...
Efficient RNA 5′-adenylation by T4 DNA ligase to facilitate practical applications
We describe a simple procedure for RNA 5′-adenylation using T4 DNA ligase. The 5′-monophosphorylated terminus of an RNA substrate is annealed to a complementary DNA strand that has a 3′-overhang of 10 nucleotides. Then, T4 DNA ligase and ATP are used to synthesize 5′-adenylated RNA (5′-AppRNA), whic...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
2006
|
| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1464850/ https://ncbi.nlm.nih.gov/pubmed/16618967 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.33106 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|