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Improvement of DNA adenylation using T4 DNA ligase with a template strand and a strategically mismatched acceptor strand
DNA with a 5′-adenylpyrophosphoryl cap (5′-adenylated DNA; AppDNA) is an activated form of DNA that is the biochemical intermediate of the reactions catalyzed by DNA ligase, RNA ligase, polynucleotide kinase, and other nucleic acid modifying enzymes. 5′-Adenylated DNA is also useful for in vitro sel...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2275898/ https://ncbi.nlm.nih.gov/pubmed/18022669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bioorg.2007.10.001 |
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