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Probing the importance of hydrogen bonds in the active site of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulation
Hydrogen bonds occurring in the catalytic triad (Asp(32), His(64) and Ser(221)) and the oxyanion hole (Asn(155)) are very important to the catalysis of peptide bond hydrolysis by serine proteases. For the subtilisin NK (nattokinase), a bacterial serine protease, construction and analysis of a three-...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Portland Press Ltd.
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1462690/ https://ncbi.nlm.nih.gov/pubmed/16411898 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20050772 |
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