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Cation-binding sites of subtilisin Carlsberg probed with Eu(III) luminescence.
Two Ca(2+)-binding sites of subtilisin Carlsberg are studied by monitoring static and time-resolved luminescence of selectively substituted Eu(3+) at each site, and they are found to be characteristically quite different from each other. Compared with the coordination sphere of free Eu(3+), two site...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2000
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1301107/ https://ncbi.nlm.nih.gov/pubmed/11023921 |
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