Directed mutagenesis of YAC-cloned DNA using a rapid, PCR-based screening protocol.
We have developed a system which facilitates the rapid modification of yeast artificial chromosome (YAC) insert DNA. Specific modifications, such as deletions, insertions and point mutations, can be generated by a two-step allele replacement method using the yeast translational suppressor, SUP4-o, a...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1996
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146091/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3467 |
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