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Selective isolation of cosmid clones by homologous recombination in Escherichia coli.

A procedure for selection of specific cosmid clones by homologous recombination between cosmid clones from a library and sequences cloned into a plasmid has been developed. Cosmid libraries constructed in a rec- host strain are packaged in vivo into lambda particles. Appropriate aliquots are then in...

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Detalhes bibliográficos
Main Authors: Poustka, A, Rackwitz, H R, Frischauf, A M, Hohn, B, Lehrach, H
Formato: Artigo
Idioma:Inglês
Publicado em: 1984
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC345382/
https://ncbi.nlm.nih.gov/pubmed/6330743
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