Selective isolation of cosmid clones by homologous recombination in Escherichia coli.

A procedure for selection of specific cosmid clones by homologous recombination between cosmid clones from a library and sequences cloned into a plasmid has been developed. Cosmid libraries constructed in a rec- host strain are packaged in vivo into lambda particles. Appropriate aliquots are then in...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Poustka, A, Rackwitz, H R, Frischauf, A M, Hohn, B, Lehrach, H
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1984
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC345382/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6330743/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.81.13.4129
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