Directed mutagenesis of YAC-cloned DNA using a rapid, PCR-based screening protocol.

We have developed a system which facilitates the rapid modification of yeast artificial chromosome (YAC) insert DNA. Specific modifications, such as deletions, insertions and point mutations, can be generated by a two-step allele replacement method using the yeast translational suppressor, SUP4-o, a...

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Detaylı Bibliyografya
Asıl Yazarlar: Tucker, R M, Burke, D T
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1996
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC146091/
https://ncbi.nlm.nih.gov/pubmed/8811104
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