Directed mutagenesis of YAC-cloned DNA using a rapid, PCR-based screening protocol.

We have developed a system which facilitates the rapid modification of yeast artificial chromosome (YAC) insert DNA. Specific modifications, such as deletions, insertions and point mutations, can be generated by a two-step allele replacement method using the yeast translational suppressor, SUP4-o, a...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Tucker, R M, Burke, D T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1996
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146091/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8811104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/24.17.3467
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