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Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage

DNA damage that leads to formation of DNA double-strand breaks (DSBs) induces phosphorylation of histone H2AX on Ser-139 at sites flanking the breakage. Immunocytochemical detection of phosphorylated H2AX (denoted as γH2AX) thus provides a marker of DSBs. The method presented in this chapter describ...

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Autors principals: Huang, Xuan, Darzynkiewicz, Zbigniew
Format: Artigo
Idioma:Inglês
Publicat: 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1458374/
https://ncbi.nlm.nih.gov/pubmed/16673875
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