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Cytometric Assessment of Histone H2AX Phosphorylation: A Reporter of DNA Damage
DNA damage that leads to formation of DNA double-strand breaks (DSBs) induces phosphorylation of histone H2AX on Ser-139 at sites flanking the breakage. Immunocytochemical detection of phosphorylated H2AX (denoted as γH2AX) thus provides a marker of DSBs. The method presented in this chapter describ...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1458374/ https://ncbi.nlm.nih.gov/pubmed/16673875 |
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