Multiplex allele-specific target amplification based on PCR suppression

We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrat...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Broude, Natalia E., Zhang, Lingang, Woodward, Karen, Englert, David, Cantor, Charles R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 2001
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC14569/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11136256/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.98.1.206
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