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Multiplex allele-specific target amplification based on PCR suppression

We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrat...

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Hlavní autoři: Broude, Natalia E., Zhang, Lingang, Woodward, Karen, Englert, David, Cantor, Charles R.
Médium: Artigo
Jazyk:Inglês
Vydáno: The National Academy of Sciences 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC14569/
https://ncbi.nlm.nih.gov/pubmed/11136256
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