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Tailoring in vitro evolution for protein affinity or stability

We describe a rapid and general technology working entirely in vitro to evolve either the affinity or the stability of ligand-binding proteins, depending on the chosen selection pressure. Tailored in vitro selection strategies based on ribosome display were combined with in vitro diversification by...

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Detalhes bibliográficos
Main Authors: Jermutus, Lutz, Honegger, Annemarie, Schwesinger, Falk, Hanes, Jozef, Plückthun, Andreas
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC14547/
https://ncbi.nlm.nih.gov/pubmed/11134506
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