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Characterization of yeast pyruvate kinase 1 as a protein kinase A substrate, and specificity of the phosphorylation site sequence in the whole protein

Pyk1 (pyruvate kinase 1) from Saccharomyces cerevisiae was characterized as a substrate for PKA (protein kinase A) from bovine heart and yeast. By designing Pyk1 synthetic peptides containing potential PKA sequence targets (Ser(22), Thr(94) and Thr(478)) we determined that the peptide S22 was a subs...

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Autors principals: Portela, Paula, Moreno, Silvia, Rossi, Silvia
Format: Artigo
Idioma:Inglês
Publicat: Portland Press Ltd. 2006
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1450003/
https://ncbi.nlm.nih.gov/pubmed/16426231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20051642
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