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Characterization of yeast pyruvate kinase 1 as a protein kinase A substrate, and specificity of the phosphorylation site sequence in the whole protein

Pyk1 (pyruvate kinase 1) from Saccharomyces cerevisiae was characterized as a substrate for PKA (protein kinase A) from bovine heart and yeast. By designing Pyk1 synthetic peptides containing potential PKA sequence targets (Ser(22), Thr(94) and Thr(478)) we determined that the peptide S22 was a subs...

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Detalhes bibliográficos
Main Authors: Portela, Paula, Moreno, Silvia, Rossi, Silvia
Formato: Artigo
Idioma:Inglês
Publicado em: Portland Press Ltd. 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1450003/
https://ncbi.nlm.nih.gov/pubmed/16426231
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20051642
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