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Distinct Roles of PI(3,4,5)P(3) during Chemoattractant Signaling in Dictyostelium: A Quantitative In Vivo Analysis by Inhibition of PI3-Kinase

The role of PI(3,4,5)P(3) in Dictyostelium signal transduction and chemotaxis was investigated using the PI3-kinase inhibitor LY294002 and pi3k-null cells. The increase of PI(3,4,5)P(3) levels after stimulation with the chemoattractant cAMP was blocked >95% by 60 μM LY294002 with half-maximal eff...

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Detalhes bibliográficos
Main Authors: Loovers, Harriët M., Postma, Marten, Keizer-Gunnink, Ineke, Huang, Yi Elaine, Devreotes, Peter N., van Haastert, Peter J.M.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1415331/
https://ncbi.nlm.nih.gov/pubmed/16421252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E05-09-0825
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