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Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

BACKGROUND: T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinom...

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Udgivet i:BMC Genomics
Principais autores: Zhao, Hongjuan, Hastie, Trevor, Whitfield, Michael L, Børresen-Dale, Anne-Lise, Jeffrey, Stefanie S
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2002
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC137577/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12445333/
https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2164-3-31
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