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Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis
BACKGROUND: T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinom...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC137577/ https://ncbi.nlm.nih.gov/pubmed/12445333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-3-31 |
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