Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

BACKGROUND: T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinom...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Zhao, Hongjuan, Hastie, Trevor, Whitfield, Michael L, Børresen-Dale, Anne-Lise, Jeffrey, Stefanie S
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: BioMed Central 2002
Gaiak:
Methodology Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC137577/
https://ncbi.nlm.nih.gov/pubmed/12445333
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-3-31
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