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Prp8p dissection reveals domain structure and protein interaction sites
We describe a novel approach to characterize the functional domains of a protein in vivo. This involves the use of a custom-built Tn5-based transposon that causes the expression of a target gene as two contiguous polypeptides. When used as a genetic screen to dissect the budding yeast PRP8 gene, thi...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Copyright 2006 by RNA Society
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370899/ https://ncbi.nlm.nih.gov/pubmed/16373487 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2281306 |
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