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Prp8p dissection reveals domain structure and protein interaction sites

We describe a novel approach to characterize the functional domains of a protein in vivo. This involves the use of a custom-built Tn5-based transposon that causes the expression of a target gene as two contiguous polypeptides. When used as a genetic screen to dissect the budding yeast PRP8 gene, thi...

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Autori principali: BOON, KUM-LOONG, NORMAN, CHRISTINE M., GRAINGER, RICHARD J., NEWMAN, ANDREW J., BEGGS, JEAN D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Copyright 2006 by RNA Society 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370899/
https://ncbi.nlm.nih.gov/pubmed/16373487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2281306
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