High-performance liquid chromatography purification of homogenous-length RNA produced by trans cleavage with a hammerhead ribozyme.

An improved method is presented for the preparation of milligram quantities of homogenous-length RNAs suitable for nuclear magnetic resonance or X-ray crystallographic structural studies. Heterogeneous-length RNA transcripts are processed with a hammerhead ribozyme to yield homogenous-length product...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Shields, T P, Mollova, E, Ste Marie, L, Hansen, M R, Pardi, A
Format: Artigo
Jezik:Inglês
Izdano: 1999
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369848/
https://ncbi.nlm.nih.gov/pubmed/10496226
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki