High-performance liquid chromatography purification of homogenous-length RNA produced by trans cleavage with a hammerhead ribozyme.

An improved method is presented for the preparation of milligram quantities of homogenous-length RNAs suitable for nuclear magnetic resonance or X-ray crystallographic structural studies. Heterogeneous-length RNA transcripts are processed with a hammerhead ribozyme to yield homogenous-length product...

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Bibliografski detalji
Glavni autori: Shields, T P, Mollova, E, Ste Marie, L, Hansen, M R, Pardi, A
Format: Artigo
Jezik:Inglês
Izdano: 1999
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1369848/
https://ncbi.nlm.nih.gov/pubmed/10496226
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