Chemical Modification of the Bacterial Porin OmpF: Gain of Selectivity by Volume Reduction

OmpF is an essentially nonselective porin isolated from the outer membrane of Escherichia coli. Here we report on the manipulation of the ion selectivity of OmpF by chemical modification with MTS reagents (MTSET, MTSEA, and MTSES) and the (rather bulky) tripeptide glutathione, all cysteine specific....

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Bibliografske podrobnosti
Main Authors: Vrouenraets, Maarten, Wierenga, Jenny, Meijberg, Wim, Miedema, Henk
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2006
Teme:
Channels, Receptors, and Electrical Signaling
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367271/
https://ncbi.nlm.nih.gov/pubmed/16299071
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.072298
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