Scalable Generation of High-Titer Recombinant Adeno-Associated Virus Type 5 in Insect Cells

We established a method for production of recombinant adeno-associated virus type 5 (rAAV5) in insect cells by use of baculovirus expression vectors. One baculovirus harbors a transgene between the inverted terminal repeat sequences of type 5, and the second expresses Rep78 and Rep52. Interestingly,...

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Detalles Bibliográficos
Main Authors: Urabe, Masashi, Nakakura, Takayo, Xin, Ke-Qin, Obara, Yoko, Mizukami, Hiroaki, Kume, Akihiro, Kotin, Robert M., Ozawa, Keiya
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2006
Assuntos:
Gene Delivery
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1367135/
https://ncbi.nlm.nih.gov/pubmed/16439543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.80.4.1874-1885.2006
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