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Amyloid Formation of a Protein in the Absence of Initial Unfolding and Destabilization of the Native State

In 5% (v/v) trifluoroethanol, pH 5.5, 25°C one of the acylphosphatases from Drosophila melanogaster (AcPDro2) forms fibrillar aggregates that bind thioflavin T and Congo red and have an extensive β-sheet structure, as revealed by circular dichroism. Atomic force microscopy indicates that the fibrils...

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Bibliografske podrobnosti
Main Authors: Soldi, Gemma, Bemporad, Francesco, Torrassa, Silvia, Relini, Annalisa, Ramazzotti, Matteo, Taddei, Niccolò, Chiti, Fabrizio
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2005
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366988/
https://ncbi.nlm.nih.gov/pubmed/16169977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.067538
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