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The Approach to the Michaelis Complex in Lactate Dehydrogenase: The Substrate Binding Pathway
We examine here the dynamics of forming the Michaelis complex of the enzyme lactate dehydrogenase by characterizing the binding kinetics and thermodynamics of oxamate (a substrate mimic) to the binary lactate dehydrogenase/NADH complex over multiple timescales, from nanoseconds to tens of millisecon...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1366705/ https://ncbi.nlm.nih.gov/pubmed/15980172 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.062604 |
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