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Structural Transformations in the Dynamics of Michaelis Complex Formation in Lactate Dehydrogenase
The dynamical nature of the binding of a substrate surrogate to lactate dehydrogenase is examined on the nanoseconds to milliseconds timescale by laser-induced temperature-jump relaxation spectroscopy. Fluorescence emission of the nicotinamide group of bound NADH is used to define the pathway and ki...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Biophysical Society
2005
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1289277/ https://ncbi.nlm.nih.gov/pubmed/15879476 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.064675 |
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