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Structural Transformations in the Dynamics of Michaelis Complex Formation in Lactate Dehydrogenase

The dynamical nature of the binding of a substrate surrogate to lactate dehydrogenase is examined on the nanoseconds to milliseconds timescale by laser-induced temperature-jump relaxation spectroscopy. Fluorescence emission of the nicotinamide group of bound NADH is used to define the pathway and ki...

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Detalhes bibliográficos
Main Authors: McClendon, Sebastian, Vu, Dung M., Clinch, Keith, Callender, Robert, Dyer, R. Brian
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1289277/
https://ncbi.nlm.nih.gov/pubmed/15879476
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.064675
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