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Specific and Nonspecific Hybridization of Oligonucleotide Probes on Microarrays

Gene expression analysis by means of microarrays is based on the sequence-specific binding of RNA to DNA oligonucleotide probes and its measurement using fluorescent labels. The binding of RNA fragments involving sequences other than the intended target is problematic because it adds a chemical back...

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Detalhes bibliográficos
Main Authors: Binder, Hans, Preibisch, Stephan
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366534/
https://ncbi.nlm.nih.gov/pubmed/15834006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.055343
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