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Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase
In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC135170/ https://ncbi.nlm.nih.gov/pubmed/12081948 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.14.3794-3800.2002 |
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