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Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase

In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Barriault, Diane, Plante, Marie-Michèle, Sylvestre, Michel
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2002
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC135170/
https://ncbi.nlm.nih.gov/pubmed/12081948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.14.3794-3800.2002
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