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Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase

In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...

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Bibliografski detalji
Glavni autori: Barriault, Diane, Plante, Marie-Michèle, Sylvestre, Michel
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2002
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC135170/
https://ncbi.nlm.nih.gov/pubmed/12081948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.14.3794-3800.2002
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