Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase

In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Barriault, Diane, Plante, Marie-Michèle, Sylvestre, Michel
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Enzymes and Proteins
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC135170/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12081948/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.184.14.3794-3800.2002
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!