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Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase

In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...

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書目詳細資料
發表在:J Bacteriol
Principais autores: Barriault, Diane, Plante, Marie-Michèle, Sylvestre, Michel
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2002
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在線閱讀:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC135170/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12081948/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.184.14.3794-3800.2002
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