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Family Shuffling of a Targeted bphA Region To Engineer Biphenyl Dioxygenase

In this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (BPDOs). Instead of shuffling the whole gene, we have targeted a fragment of bphA that is critical for enzyme specificity. We also describe...

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Detalhes bibliográficos
Main Authors: Barriault, Diane, Plante, Marie-Michèle, Sylvestre, Michel
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC135170/
https://ncbi.nlm.nih.gov/pubmed/12081948
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.14.3794-3800.2002
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