Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces

Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Lee, Li-Fong, Yeh, Shun-Hua, Chen, Carton W.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Genetics and Molecular Biology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11807086/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.184.4.1214-1218.2002
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