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Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces

Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to...

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Publicado en:J Bacteriol
Autores principales: Lee, Li-Fong, Yeh, Shun-Hua, Chen, Carton W.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2002
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134811/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11807086/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.184.4.1214-1218.2002
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