Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134811/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11807086/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.184.4.1214-1218.2002 |
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