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Construction and Synchronization of dnaA Temperature-Sensitive Mutants of Streptomyces
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39°C replication in the mutants ceased in about 90 min but resumed on return to...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
American Society for Microbiology
2002
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC134811/ https://ncbi.nlm.nih.gov/pubmed/11807086 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/jb.184.4.1214-1218.2002 |
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