載入...

Transduction-Mediated Transfer of Unmarked Deletion and Point Mutations through Use of Counterselectable Suicide Vectors

A challenge in strain construction is that unmarked deletion and nucleotide substitution alleles generally do not confer selectable phenotypes. We describe here a rapid and efficient strategy for transferring such alleles via generalized transduction. The desired allele is first constructed and intr...

全面介紹

Na minha lista:
書目詳細資料
Main Authors: Kang, Ho Young, Dozois, Charles M., Tinge, Steven A., Lee, Tae Ho, Curtiss III, Roy
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2002
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC134762/
https://ncbi.nlm.nih.gov/pubmed/11741872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.184.1.307-312.2002
標簽: 添加標簽
沒有標簽, 成為第一個標記此記錄!