Specialized Transduction Designed for Precise High-Throughput Unmarked Deletions in Mycobacterium tuberculosis

Specialized transduction has proven to be useful for generating deletion mutants in most mycobacteria, including virulent Mycobacterium tuberculosis. We have improved this system by developing (i) a single-step strategy for the construction of allelic exchange substrates (AES), (ii) a temperature-se...

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Detalhes bibliográficos
Main Authors: Jain, Paras, Hsu, Tsungda, Arai, Masayoshi, Biermann, Karolin, Thaler, David S., Nguyen, Andrew, González, Pablo A., Tufariello, Joann M., Kriakov, Jordan, Chen, Bing, Larsen, Michelle H., Jacobs, William R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Microbiology 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049104/
https://ncbi.nlm.nih.gov/pubmed/24895308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.01245-14
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