Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays

Hybridization kinetics were found to be significantly different for specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non- spec...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Dai, Hongyue, Meyer, Michael, Stepaniants, Sergey, Ziman, Michael, Stoughton, Roland
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2002
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134259/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12177314/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnf085
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