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Selection of primer-template sequences that bind human immunodeficiency virus reverse transcriptase with high affinity
A SELEX (systematic evolution of ligands by exponential enrichment)-based approach was developed to determine whether HIV-RT showed preference for particular primer-template sequences. A 70 nt duplex DNA was designed with 20 nt fixed flanking sequences at the 3′ and 5′ ends and a randomized 30 nt in...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1325207/ https://ncbi.nlm.nih.gov/pubmed/16397296 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkj426 |
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