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Selection of primer-template sequences that bind human immunodeficiency virus reverse transcriptase with high affinity

A SELEX (systematic evolution of ligands by exponential enrichment)-based approach was developed to determine whether HIV-RT showed preference for particular primer-template sequences. A 70 nt duplex DNA was designed with 20 nt fixed flanking sequences at the 3′ and 5′ ends and a randomized 30 nt in...

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Bibliografski detalji
Glavni autori: DeStefano, Jeffrey J., Cristofaro, Jason V.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2006
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1325207/
https://ncbi.nlm.nih.gov/pubmed/16397296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkj426
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