Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector

We have designed a doxycycline-regulated form of the H1 promoter of RNA polymerase III that allows the inducible knockdown of gene expression by small interfering RNAs (siRNAs). As a proof-of-principle, we have targeted β-catenin in colorectal cancer (CRC) cells. T-cell factor (TCF) target-gene expr...

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Hlavní autoři: van de Wetering, Marc, Oving, Irma, Muncan, Vanesa, Pon Fong, Menno Tjon, Brantjes, Helen, van Leenen, Dik, Holstege, Frank C.P., Brummelkamp, Thijn R., Agami, Reuven, Clevers, Hans
Médium: Artigo
Jazyk:Inglês
Vydáno: 2003
Témata:
Scientific Report
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1319205/
https://ncbi.nlm.nih.gov/pubmed/12776180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.embor.embor865
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