Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila in Clinical Specimens by Dual-Color Real-Time PCR and Melting Curve Analysis

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory...

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Bibliographische Detailangaben
Hauptverfasser: Reischl, Udo, Linde, Hans-Jörg, Lehn, Norbert, Landt, Olfert, Barratt, Kevin, Wellinghausen, Nele
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2002
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC130908/
https://ncbi.nlm.nih.gov/pubmed/12354888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.10.3814-3817.2002
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