Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila in Clinical Specimens by Dual-Color Real-Time PCR and Melting Curve Analysis

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory...

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Detalles Bibliográficos
Main Authors: Reischl, Udo, Linde, Hans-Jörg, Lehn, Norbert, Landt, Olfert, Barratt, Kevin, Wellinghausen, Nele
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2002
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC130908/
https://ncbi.nlm.nih.gov/pubmed/12354888
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.10.3814-3817.2002
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