Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila in Clinical Specimens by Dual-Color Real-Time PCR and Melting Curve Analysis

A dual-color LightCycler PCR assay targeting the 16S rDNA gene of Legionella spp. was established. By using two pairs of hybridization probes, Legionella spp. and Legionella pneumophila could be detected and differentiated simultaneously. With 26 culture-positive and 42 culture-negative respiratory...

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Vydáno v:	J Clin Microbiol
Hlavní autoři:	Reischl, Udo, Linde, Hans-Jörg, Lehn, Norbert, Landt, Olfert, Barratt, Kevin, Wellinghausen, Nele
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 2002
Témata:	Bacteriology
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC130908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12354888/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.10.3814-3817.2002
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