Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-ba...

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Huvudupphovsmän: Jaulhac, B., Reyrolle, M., Sodahlon, Y. K., Jarraud, S., Kubina, M., Monteil, H., Piémont, Y., Etienne, J.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1998
Ämnen:
Bacteriology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC104998/
https://ncbi.nlm.nih.gov/pubmed/9650980
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