Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-ba...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Jaulhac, B., Reyrolle, M., Sodahlon, Y. K., Jarraud, S., Kubina, M., Monteil, H., Piémont, Y., Etienne, J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	Bacteriology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC104998/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9650980/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.36.7.2120-2122.1998
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