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Imaging the Activity and Localization of Single Voltage-Gated Ca(2+) Channels by Total Internal Reflection Fluorescence Microscopy

The patch-clamp technique has enabled functional studies of single ion channels, but suffers limitations including lack of spatial information and inability to independently monitor currents from more than one channel. Here, we describe the use of total internal reflection fluorescence microscopy as...

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Bibliographic Details
Main Authors: Demuro, Angelo, Parker, Ian
Format: Artigo
Language:Inglês
Published: Biophysical Society 2004
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304190/
https://ncbi.nlm.nih.gov/pubmed/15111438
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